ÖZET
Amaç:
Mevcut araştırma, farklı çözücülerle hazırlanan Pyracantha coccinea Roem. meyve ekstraktlarının toplam flavonoid içeriği, toplam fenolik içeriği, ağır metal bileşimi ve antioksidan ve antimikrobiyal aktivitelerini değerlendirmek için yapılmıştır.
Yöntemler:
Pyracantha coccinea Roem. meyvesinin ekstraktını hazırlamak için çözücü olarak etanol, dietil eter ve sıcak su ekstraksiyonu kullanıldı. Toplam fenolik bileşen Folin-Ciocalteu yöntemi ile toplam flavonoidlerin içerik AlCl3 yöntemi ile spektrofotometrik olarak ölçüldü. Ekstraktların antioksidan aktiviteleri, serbest radikal süpürme deneyleri, DPPH ve ABTS ile araştırıldı. Meyveler, ağır metal içeriğini belirlemek için İndüktif Eşleşmiş Plazma-Kütle Spektrometresi ile analiz edildi. Ekstraktların antimikrobiyal aktiviteleri, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus ve Candida albicans’a karşı agar kuyusu difüzyon yöntemi kullanılarak araştırıldı.
Bulgular:
Sıcak su ekstraktının toplam flavonoid bileşeninin, toplam fenolik bileşeninin, ABTS ve DPPH aktivitelerinin diğer fraksiyonlara göre anlamlı derecede yüksek olduğu belirlendi. Bu parametrelerin etanol ekstraktında eter ekstraktından önemli ölçüde daha yüksek olduğu bulundu. Tüm ekstreler Bacillus cereus ve Pseudomonas aeruginosa’ya karşı antimikrobiyal aktivite sergilerken, sıcak su fraksiyonu Pseudomonas aeruginosa’ya karşı en yüksek antibakteriyel etkiyi sergiledi. Cr, Co, Ni ve Cu içeriklerinin bitkilerde bulunabilecek toksisite eşiklerini aştığı belirlendi.
Sonuç:
Bu sonuçlar, Pyracantha coccinea Roem. meyvesinin doğal bir antioksidan ve antimikrobiyal ajan kaynağı olarak kabul edilebileceğini göstermektedir.
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