ÖZET
Amaç:
Kolon kanserinin önlenmesinde potansiyel bir anti-kanser ajan olarak kabul edilen kersetin, doğal polifenolik bileşiklerinden biridir. Hücrelerden ve bileşenlerinden salgılanan eksozom gibi hücre dışı veziküller, proteinleri veya miRNA’ları taşıma yoluyla hücresel davranış özelliklerine katkıda bulunur. Bu çalışmada, kersetin uygulanan Colo320 ve Colo741 kolon kanseri hücre dizilerinde Dicer, Ago2, eIF2α CD9 ve CD63’ün sitotoksisitesini ve eksozomal miRNA salgılanması ve ekspresyonu üzerine etkilerini belirlemeyi amaçladık.
Yöntemler:
Kersetin sitotoksisitesinin analizi için MTT testi kullanıldı. Colo320 ve Colo741’de Dicer, Ago2, eIF2a, CD9 ve CD63’ün dağıtımı için dolaylı immünoperoksidaz boyaması kullanıldı. Eksozomdaki toplam miRNA, miRCURY™ Kit ile belirlendi.
Bulgular:
Kersetin uygulamasından sonra eIF2a ve CD9 immünoreaktiviteleri Colo741 kontrol grubuna kıyasla önemli ölçüde farklılık gösterdi. Ayrıca eksozomal miRNA konsantrasyonları hem kersetin uygulanan Colo320 hem de Colo741 hücrelerinde daha yüksekti.
Sonuç:
Kersetin uygulamasının Colo320 ve Colo741 hücrelerinde eksozomal salgılamayı tetiklediği sonucuna vardık. Bununla birlikte, primer ve metastatik kolon adenokarsinom hücrelerinde kersetin rolünü anlamak için gelecekteki araştırmalarda eksozomal bileşen değerlendirilmelidir.
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