ÖZET
Amaç:
Çalışmamızda Gaziantep şehrinden toplanan Rhus coriaria L. (sumak) türünün meyvelerine ait ekstrelerin kimyasal analizi yapılarak, antioksidan ve antimikrobiyal aktivitelerinin incelenmesi amaçlanmıştır.
Yöntemler:
Rhus coriaria meyvelerinden %80 (R2) ve %100’lük (R3) etanol ekstraktları hazırlanmıştır. Ekstraktların kimyasal analizi sıvı kromatografi-yüksek çözünürlüklü kütle spektrometre yöntemi, antioksidan aktiviteleri 1,1-diphenyl-2-picrylhydrazyl (DPPH) serbest radikal giderim aktivitesi, antimikrobiyal etkinliği sıvı mikrodilüsyon yöntemi kullanılarak araştırılmıştır.
Bulgular:
R2 ve R3 ekstraktlarının kimyasal analizinde en yoğun miktarda bir organik asit olan fumarik asit sırasıyla 31076,55 ve 23348,37 mg/kg olarak saptanmıştır. R2’de konsantrasyonu en yüksek olan fenolik bileşenler hyperoside (622,24 mg/kg), ellagic acid (343,63 mg/kg) ve p-coumaric acid (182,91 mg/kg) iken, R3’te konsantrasyonu en yüksek olan fenolik bileşenlerin ellagic acid (607,30 mg/kg), hyperoside (440,41 mg/kg) ve p-coumaric acid (178,61 mg/kg) olduğu gözlenmiştir. R2 ve R3 ekstraktlarının antioksidan aktivitelerinde DPPH serbest radikal giderim aktivitesinin sırasıyla %70,78±0,002 ve %11,19±0,001 olduğu tespit edilmiştir. R2 ve R3 ekstraktlarının antimikrobiyal aktiviteleri; S. aureus ATCC 25923 kökeninde sırasıyla 125 ve <3,9 µg/mL, A. baumannii ATCC 19606 kökeninde sırasıyla 15,625 ve 31,25 µg/mL, H. pylori ATCC 43504 kökeninde 62,5 µg/mL, C. glabrata ATCC 2001 kökeninde 62,5 µg/mL ve C. albicans ATCC 66027 kökeninde <3,9 µg/mL olarak saptanmıştır.
Sonuç:
Çalışmamızda ülkemizde yetişen R. coriaria meyvelerinden elde edilen R2 ekstresinde daha yüksek saptanan antioksidan aktivitenin, R3 ekstresine kıyasla fenolik bileşen içeriğinin daha fazla olmasından kaynaklanabilir. R3 ekstresinde saptanan daha etkin antimikrobiyal aktivitenin, R2 ekstresine kıyasla fazla miktarda ellagic acid içermesinden dolayı olabileceği düşünülmüştür.
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