ÖZET
Amaç:
Mevcut çalışmada, hassas bir ultra hızlı sıvı kromatografisi yöntemine ve dansil klorür (DNS-Cl) ile türevlendirmeye dayalı olarak acı biber soslarındaki kapsaisinoidlerin miktar tayini tanımlanmaktadır. Kapsaisinoidler, biber sosları tarafından sekonder (ikincil) metabolitler olarak biyosentezlenir. Kapsaisinoidlerin ana bileşenleri kapsaisin (CPS) ve dihidrokapsaisindir (DCPS).
Yöntemler:
CPS ve DCPS içindeki fenol grupları, pH 10’da DNS-Cl (kimyasal olarak 5-(dimetilamino) naftalen-1-sülfonil klorür olarak adlandırılır) kullanılarak 0,5 M sodyum bikarbonat ile türevlendirme reaksiyonu için uygundur, bu da yüksek floresan özelliklere sahip bir türevin oluşumuna yol açar ve 360 nm dalga boyunda uyarmayı takiben 520 nm’de ölçülebilir. Bileşiklerin ayrılması, asetik asit (0,5 M, NaOH ile pH 7,0) çözeltisi ve asetonitrilin bir kombinasyonu ile oluşturulan bir mobil faza sahip kromatografik bir sistem üzerinde C18 kolonu kullanılarak, 0,4 mL dk-1 akış hızında solvent programlaması altında tutarlı bir şekilde gerçekleştirildi.
Bulgular:
Metot validasyonu, Uluslararası Harmonizasyon Topluluğu Konferansı’nda açıklanan düzenlemelere göre değerlendirildi. CPS ve DCPS için kalibrasyon grafiği 0,2 ve 200 μg mL-1 arasında doğrusaldı.
Sonuç:
Önerilen analitik prosedür, biber soslarında kapsaisinoidlerin miktar tayini ile ilgili olarak uygun bir seçiciliğe sahip, basit, zaman ve maliyet açısından etkin bir yöntemi temsil etmektedir.
References
1
Ornelas-Paz J, Martínez-Burrola JM, Ruiz-Cruz S, Santana-Rodríguez V, Ibarra-Junquera V, et al. Effect of cooking on the capsaicinoids and phenolics contents of Mexican peppers. Food Chem 2010;119:1619-25.
2
Cisneros-Pineda O, Torres-Tapia LW, Gutiérrez-Pacheco LC, ContrerasMartı´n F, Gonza´lez-Estrada T, Peraza-Sa´nche SR. Capsaicinoids quantification in chili peppers cultivated in the state of Yucatan, Mexico. Food Chem 2007;104:1755-60.
3
Constant HL, Cordell GA, West DP, Johnson JH. Separation and quantification of capsaicinoids using complexation chromatography. J Nat Prod 1995;58:1925-8.
4
CREDOC/OCA (Observatoire des Consommations Alimentaires). Estimation des niveaux d’ingestion de substances aromatisantes safrole, estragole, coumarine et capsaicine, Note Tech 1998:98;25.
5
The Capsaicin Study Group. Treatment of painful diabetic neuropathy with topical capsaicin. A multicenter, double-blind, vehiclecontrolled study. Archives of Internal Med 1991;151:2225-9.
6
Dray A. Neuropharmacological mechanisms of capsaicin and related substances. Biochem. Pharmacology 1992:44;611-5.
7
Mozsik G, Domotor A, Past T, VasV, Perjesi P, Kuzma M, et al. Capsaicinoids. Academy Publisher. 2009; Budapest/Hungary: pp. 1-255.
8
Mozsik G, Past T, Abdel-Salam OM, Kuzma M, Perjesi P. Interdisciplinary review for correlation between the plant origin capsaicinoids, non-steroidal antiinflammatory drugs, gastrointestinal mucosal damage and prevention in animals and human beings. Inflammopharmacology 2009:17;113-50.
9
Hassan MH, Edfawy M, Mansour A, Hamed AA. Antioxidant and antiapoptotic effects of capsaicin against carbon tetrachloride-induced hepatotoxicity in rats. Toxicol Ind Health 2012:28;428-38.
10
Henderson DE, Slickman AM, Henderson SK. Quantitative HPLC determination of the antioxidant activity of capsaicin on the formation of lipid hydroperoxides of linoleic acid: a comparative study against BHT and melatonin. J Agric Food Chem 1999:47;2563-70.
11
Bhutani M, Pathak AK, Nair AS, Kunnumakkara AB, Guha S, Sethi G, Aggarwal BB. Capsaicin is a novel blocker of constitutive and interleukin-6-inducible stat3 activation. Clin Cancer Res 2007:13;3024-32.
12
Huang SP, Chen JC, Wu CC, Chen CT, Tang NY, Ho YT, et al. Capsaicin-induced apoptosis in human hepatoma hepg2 cells. Anticancer Res 2009:29;165-74.
13
Morris GC, Gibson SJ, Helme RD. Capsaicin-inducedflare and vasodilatation in patients with post-herpetic neuralgia. Pain 1995:63;93-101.
14
Kaale EA, Schepdael AV, Roets E, Hoogmartens J. Determination of capsaicinoids in topical cream by liquid-liquid extraction and liquid chromatography. J Pharmaceut Biomed Anal 2002:30;1331-7.
15
Higashiguchi F, Nakamura H, Hayashi H, Kometani T. Purification and Structure Determination of Glucosides of Capsaicin and Dihydrocapsaicin from Various Capsicum Fruits. J Agric Food Chem 2006:54;5948-53.
16
Schweiggert U, Carle R, Schieber A. Characterization of major and minor capsaicinoids and related compounds in chili pods (Capsicum frutescens L.) by high-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry. Anal Chim ActA 2006:557;236-44.
17
Kozukue N, Han JS, Kozukue E, Lee SJ, Kim JA, Lee KR, et al. Analysis of eight capsaicinoids in peppers and pepper-containing foods by high-performance liquid chromatography and liquid chromatographymass spectrometry. J Agric Food Chem 2005:53;9172-918.
18
Peña-Alvarez A, Ramírez-Maya E, Alvarado-Suárez LA. Analysis of capsaicin and dihydrocapsaicin in peppers and pepper sauces by solid phase microextraction-gas chromatography-mass spectrometry. J Chromatogr A 2009:1216;2843-7.
19
Laskaridou-monnerville A. Determination of capsaicin and dihydrocapsaicin by micellar electrokinetic capillary chromatography and its application to various species of Capsicum. J Chromatogr A 1999:838;293-302.
20
Berke TG, Shieh SC. In Handbook of Herbs and Spices . ed. K. V. Peter, 267 CRC Press LLC, Boca Raton, 1, 2001. p. 220.
21
Weber G. Polarization of the fluorescence of macromolecules. 2. Fluorescent conjugates of ovalbumin and bovine serum albumin. Biochem J 1952:51;155-67.
22
El Sayed L, Mohamed TA, Taha EA. Spectrofluorimetric determination of carvedilol in dosage form and spiked human plasma through derivatization with 1-dimethylamino-naphthalene-5-sulphonyl chloride. Chem Ind Chem Eng Q 2010:16;31-8.
23
Penzes LP, Oertel GW. Determination of steroids by densitometry of derivatives. II. Direct fluorometry of dansyl estrogens. J Chromatogr.1970:51;325-7.
24
Roos RW. High pressure liquid chromatographic analysis of estrogens in pharmaceuticals by measurement of their dansyl derivatives. J Pharm Sci 1978:67;1735-9.
25
Wang X, Liang Y, Wang Y, Fan M, Sun Y, Liu J, et al. Simultaneous determination of 10 kinds of biogenic amines in rat plasma using high-performance liquid chromatography coupled with fluorescence detection. Biomed Chromatogr 2018:32;4211.
26
Tekkeli Kepekci S.E. Spectrofluorimetric Method for the Determination of Tolterodine In Human Plasma and Pharmaceutical Preparations by Derivatization with Dansyl Chloride. Bezmialem Science 2017:5;50-55.
27
Sagıroglu AA, Onal C, Tekkeli SE. Liquid chromatographic analysis for the determination of deferasirox in pharmaceutical formulations and spiked plasma samples using dansyl chloride reagent. J Chem Metrol 2020:14;35-41.
28
Onal C, Tekkeli SE. Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent. J Res Pharm 2020:24;765-73.
29
Guideline IHT. Validation of Analytical Procedures. Text and Methodology, Q2 (R1). 2005.
30
Ha J, Seo HY, Shim YS, Seo DW, Seog H, Ito M, et al. Determination of capsaicinoids in foods using ultra high performance liquid chromatography. Food Sci Biotechnol 2010:19;1005-9.
