Öz
Amaç: Yeni keşfedilmiş endemik bir tür olan Seseli salsugineum
(S. salsugineum) A. Duran and Lyskov’un toprak üstü kısımlarından elde edilen uçucu yağın kimyasal bileşimi ve antimikrobiyal aktivitesinin ilk kez incelenmesi amaçlanmıştır.
Yöntemler: S. salsugineum’un toprak üstü kısımlarının Clevenger apareyi ile uçucu yağı elde edilmiş ve uçucu yağın bileşimi gaz kromatografisi (GK) ve GK-kütle spektrometresi (GK-KS) ile belirlenmiştir. Uçucu yağın antimikrobiyal aktivitesi mikrodilüsyon yöntemi ile Gram-negatif (Pseudomonas aeruginosa ATCC B888), Gram-pozitif (Staphylococcus aureus ATCC 6538, Bacillus cereus NRRL B-3711), ve üç fungus suşu: Candida albicans ATCC 24433, Candida parapsilosis ATCC 22019 ve Candida krusei ATCC 6258 suşlarına karşı çalışılmıştır. Pozitif kontrol olarak kloramfenikol ve amfoterisin B kullanılmış ve minimum inhibisyon konsantrasyonları belirlenmiştir.
Bulgular: S. salsugineum uçucu yağı verimi %0,28’dir (h/a). GK ve GK-KS analizinde uçucu yağın ana bileşenler olarak sabinen (%35,5), kessan (%10,5), α-pinen (%6,4) ve terpinen-4-ol (%5,0) bulunmuştur. Uçucu yağ test edilen tüm suşlara karşı (320-1280 µg/mL) antimikrobiyal aktivite göstermiştir.
Sonuç: Bildiğimiz kadarıyla, bu çalışma ile S. salsugineum uçucu yağının kimyası ve biyolojik aktivitesi ilk kez çalışılmıştır.
Anahtar Kelimeler: Seseli salsugineum, Apiaceae, uçucu yağ, antibakteriyel aktivite, antifungal aktivite
Kaynakça
Pimenov MG, Leonov MV. The Asian Umbelliferae biodiversity database (ASIUM) with particular reference to south-west Asian taxa. Turk J Bot. 2004;28:139-45.
Davis PH. Flora of Turkey and the East Aegean Islands. Edinburgh: Edinburgh University Press; 1988.
Tosun A, Özkal N. Chemical constituents and biological activities ofSeseli L. (Umbelliferae) species. J Fac Pharm Ankara. 2003;32:269-84.
Duran A, Samigullin T, Lyskov D.Seseli salsugineum (Apiaceae), A new species from Central Anatolia, Turkey. Phytotaxa. 2021;529:27-42.
Önder A, Gurpınar SS, Eryilmaz M, Akay BK, Çınar AS. Evaluation of antimicrobial potential of some spices from Apiaceae sold in herbal shops. Journal of Medicinal Botany. 2019;3:1-4.
Doğan Güner E, Duman H, Pınar NM. Pollen morphology of the genusSeseli L. (Umbelliferae) in Turkey. Turk J Bot. 2011;35:175-82.
Önder A, Nahar L, Çınar AS, Sarker SD. The genusSeseli L.: A comprehensive review on traditional uses, phytochemistry, and pharmacological properties. J Herb Med.2023;38:100625.
Banerjee SK, Gupta, BD, Kumar R, Atal CK. New coumarins from the umbels of Seselisibiricum. Phytochemistry.;19:281-4.
Janaćković P, Soković M, Vujisić L, Vajs V, Vucković I, Krivosej Z, et al. Composition and antimicrobial activity of Seseli globiferum essential oil. Nat Prod Commun. 2011;6:1163-6.
Bulatović VM, Šavikin-Fodulović KP, Zdunić GM, Popović MP. Essential oil ofSeseli peucedanoides (MB) Kos.-Pol. J Essent Oil Res. 2006;18:286-7.
Milosavljević S, Tesević V, Vucković I, Jadranin M, Vajs V, Soković M, et al. Composition and antifungal activity of the essential oil ofSeseliannuum wild-growing in Serbia. Fitoterapia. 2007;78:319-22.
Doğan E, Duman H, Tosun A, Kurkcuoglu M, Baser KHC. Essential oil composition of the fruits ofSeseli resinosum Freyn et Sint. and Seseli tortuosum L. growing in Turkey.JEOR. 2006;18:57-9.
Tosun A, Chun J, Jerković I, Marijanović Z, Fenu MA, Aslan SS, et al. Chemical profiles and anti-inflammatory activity of the essential oils fromSeseligummiferum and Seseli corymbosum subsp. corymbosum. NPC. 2016;11:1523-6.
Tosun A. Occurrence of coumarins inSeselihartvigii growing in Turkey. Chem Nat Compound. 2006;42:608-9.
Küpeli E, Tosun A, Yesilada E. Anti-inflammatory and antinociceptive activities of Seseli L. species (Apiaceae) growing in Turkey. J Ethnopharmacol. 2006;104:310-4.
Hu CQ, Chang JJ, Lee KH. Antitumor agents, 115 Seselidiol, a new cytotoxic polyacetylene fromSeseli mairei. J Nat Prod. 1990;53:932-5.
Cinar AS, Bakar-Ates F, Onder A. Seseli petraeum M. Bieb. (Apiaceae) Significantly Inhibited Cellular Growth of A549 Lung Cancer Cells through G0/G1 Cell Cycle Arrest. An Acad Bras Cienc. 2020;92:E20191533.
Önder A, Cinar AS, Bakar-Ateş F, Noguera-Artiaga L, Carbonell-Barrachina A. Chemical composition and cytotoxic potency of essential oil fromSeseli petraeum M. Bieb. (Apiaceae). J Pharm Res. 2021;25:249-7.
Adams M, Berset C, Kessler M, Hamburger M. Medicinal herbs for the treatment of rheumatic disorders--a survey of European herbals from the 16th and 17th century. J Ethnopharmacol. 2009;121:343-59.
Önder A, Çinar AS, Yilmaz Sarialtin S, Izgi MN, Çoban T. Evaluation of the Antioxidant Potency ofSeseli L. Species (Apiaceae). Turk J Pharm Sci. 2020;17:197-202.
Demirci F, Bayramiç P, Göger G, Demirci B, Başer KHC. Characterization and antimicrobial evaluation of the essential oil ofPinus pinea L. from Turkey. Nat Volatiles Essent Oils. 2015;2:39-44.
Mc Lafferty FW, Stauffer DB. The Wiley/NBS Registry of Mass Spectral Data. New York: J Wiley and Sons; 1989.
Koenig WA, Joulain D, Hochmuth DH. Terpenoids and related constituents of essential oils. Hamburg: MassFinder 4, 2004.
CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, CLSI M7-A7, Pennsylvania: Clinical and Laboratuary Standarts Instittute; 2006.
Ilic M, Jovanovic VS, Mitic V, Jovanovic OP, Mihajilov-Krstev T, Markovic M, et al. Comparison of chemical composition and biological activities ofSeseli rigidum fruit essential oils from Serbia. Open Chem. 2015;13:42-51.
Goncalves MJ, Tavares AC, Cavaleiro C, Cruz MT, Lopes MC, Canhoto J, et al. Composition, antifungal activity and cytotoxicity of the essential oils ofSeseli tortuosum L. and Seseli montanum subsp. peixotoanum (Samp.) M. Laínz from Portugal. Industrial Crops & Products. 2012;39:204-9.
Stankov-Jovanović VP, Ilić MD, Mitić VD, Mihajilov-Krstev TM, Simonović SR, Nikolić Mandić SD, et al. Secondary metabolites ofSeseli rigidum: Chemical composition plus antioxidant, antimicrobial and cholinesterase inhibition activity. J Pharm Biomed Anal. 2015;111:78-90.
Tosun A, Baba M, Okuyama, T. HPLC analysis of coumarins in TurkishSeseli species (Umbelliferae). J Nat Med. 2007;61:402-5.
