Öz
Amaç
Elektroporasyon (EP), elektrik darbeleri uygulanarak membran geçirgenliğinin artırıldığı bir yöntemdir. EP sonrası hücrelerde değişen nabız parametreleriyle meydana gelen değişikliklerin belirlenmesi, EP teorisinin temellerinin oluşturulmasında büyük önem taşımaktadır. Bu nedenle, EP’ye tabi tutulan insan rahim ağzı kanseri hücre dizisi (HeLa) hücrelerinin zarı üzerinde gözenek oluşumu olgusuna ilişkin açıklama aradık.
Yöntemler
EP’ye bağlı olarak hücre zarında oluşan gözenekler taramalı elektron mikroskobu kullanılarak gözlemlendi. Hücrelerin canlılığı ve mitokondriyal membran potansiyelindeki (ΔΨm) değişiklik, WST-8 ve JC-1 analizleri ile belirlendi.
Bulgular
EP’ye tabi tutulmuş hücre zarının yüzeyi nispeten tekdüze bir gözenek popülasyonu sergiledi. HeLa hücrelerinin canlılığı, artan elektrik alan yoğunluklarıyla önemli ölçüde azaldı. Kontrol grubu ile 0,8 ve 1,6 kV/cm EP grupları arasında ΔΨm’de hafif bir azalma gözlendi ancak 2,4 ve 3,2 kV/cm EP gruplarında ΔΨm kontrol grubuna göre daha yüksekti.
Sonuç
Sonuç olarak çalışmamız, EP’nin rahim ağzı kanseri hücre hattına uygulanmasının, membran üzerinde değişen boyutlarda gözeneklerin oluşmasına neden olduğunu gösterdi. Elektrik alan genliğinin artmasıyla hücre canlılığı azalırken, EP tedavisi ve kontrol grupları arasında ΔΨm’de anlamlı bir değişiklik gözlenmedi. EP’ye tabi tutulmuş hücrelerdeki gözenek dağılımlarını ve farklı elektrik alanı genliklerinde ortaya çıkan değişiklikleri belirlemek için daha fazla araştırmaya ihtiyaç olduğu unutulmamalıdır.
Anahtar Kelimeler: Elektroporasyon, rahim ağzı kanseri, gözenek boyutu, hücre canlılığı, mitokondri membran potansiyeli
Kaynakça
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