İnsülinoma INS-1 Hücrelerinde Hücre Çoğalması, Apoptoz ve Oksidatif Stres Üzerine <i>Camellia sinensis</i> Ekstraktının Etkileri
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P: 80-85
Nisan 2019

İnsülinoma INS-1 Hücrelerinde Hücre Çoğalması, Apoptoz ve Oksidatif Stres Üzerine Camellia sinensis Ekstraktının Etkileri

Bezmialem Science 2019;7(2):80-85
Bilgi mevcut değil.
Bilgi mevcut değil
Alındığı Tarih: 14.12.2017
Kabul Tarihi: 02.04.2018
Yayın Tarihi: 10.05.2019
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Amaç:

Yeşil çay (Camellia sinensis) dünyanın en çok tüketilen içeceklerinden biridir. C. sinensis tüketimi tümör ve diğer hastalıkları önleyebilir. İnsülinomlar pankreatik ada hücre tümörleridir. Bu çalışmada, sıçan insülinoma INS-1 hücrelerinde C. sinensis ekstraktının etkileri araştırılmıştır.

Yöntemler:

C. sinensis yaprakları Türkiye’de Rize’de toplandı. Yapraklar 80°C’de 20 ve 40 dakika musluk suyunda demlendi. INS-1 hücreleri, 24 saat boyunca C. sinensis ekstraktının farklı dozları (1-5 mg/mL) ile muamele edildi. Hücre canlılığı, çoğalması, ölümü incelendi. C. sinensis ekstraktı verilen INS-1 hücrelerinde malondialdehit, glutatyon ve protein karbonil seviyeleri ölçüldü.

Bulgular:

C. sinensis ekstraktı ile muamele edilen INS-1 hücrelerinde kontrol grubuna göre hücre canlılığı ve PCNA immünpozitif hücrelerin yüzdesi azalırken, TUNEL pozitif hücrelerin yüzdesi artmıştır. C. sinensis ekstraktı ile muamele edilen INS-1 hücrelerinde kontrol grubuna göre malondialdehit ve protein karbonil seviyeleri azalırken, glutatyon seviyeleri artmıştır.

Sonuç:

Sonuçlar INS-1 hücresinde C. sinensis ektraktının çoğalmayı inhibe ettiğini ve apoptoza neden olduğunu göstermektedir. C. sinensis, pankreas tümörlerinin tedavisini destekleyen doğal bir ajan olabilir.

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