ÖZET
Amaç:
Glutamat, beyindeki majör uyarıcı transmiterdir. Fazla glutamat eksitotoksisiteye yol açar. GLT-1 (Glutamat Transportır 1) beyindeki glutamatın %95’ini temizleyerek nöron fonksiyonunu sağlar ve eksitotoksisiteyi önler. Bu çalışmada kainik asit and MPP+ (1-methyl-4-phenylpyridinium) isimli iki toksinin nöroblastoma ve glia (immortalize edilmiş insan astrositleri) hücrelerinde GLT-1 ve eksitotoksisite üzerindeki etkilerini incelemektir.
Yöntemler:
Nöroblastoma ve glia hücreleri kainik asit ve MPP+ ile muamele edilip hücre canlılığını ölçüldü. GLT-1’in mRNA ve protein ifadesi tespit edildikten sonra glutamat assay ile birikmiş Glutamate miktarı belirlendi.
Bulgular:
GLT-1’in mRNA seviyesi kainik asit ve MPP+ ile muamele sonucu nöroblastoma ve glia hücrelerinde artmıştır. GLT- 1’in protein seviyesi de glia hücrelerinde MPP+ muamelesi sonucu artmıştır. Salınan glutamatın 12 saatlik MPP+ muamelesinden sonra azaldığı gözlenmiştir. Ancak bu azalış daha sonra geçerli olmamıştır.
Sonuç:
Bulgularımız göstermektedir ki GLT-1 ifadesi kainik asit veya MPP+ muamelesi sonucu yaşamsal bir mekanizma olarak eksitotoksisiteyi önlemek için artmaktadır.
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