ÖZET
Amaç:
Vitamin A’nın bir retinoid türevi olan izotretinoinin (ISR) spermatogenez üzerindeki etkisinin follikül stimüle edici hormon (FSH), luteinize edici hormon (LH) ve testosteron seviyeleri üzerinden araştırılması amaçlandı.
Yöntemler:
Bu çalışma 12 adet Balb/c cinsi 3 aylık erkek fare ile gerçekleştirildi. Deney grupları kontrol (n=6) ve ISR (n=6) olmak üzere ikiye ayrıldı. Serum örneklerinde FSH, LH ve testosteron seviyeleri ELISA yöntemiyle belirlendi. Tüm analizler ve grafikler için SPSS 20,00 (IBM, New York, USA) ve Graph Pad Prism Version 6,01 (USA) programları kullanıldı. İstatistiki karşılaştırılmalarda parametrik student t-testinden yararlanıldı. Anlamlılık seviyesi p≤0,05 olarak kabul edildi.
Bulgular:
ISR grubunda ortalama FSH, LH ve testosteron seviyelerinde kontrol grubuna göre artış belirlendi. Bu artış her üç hormon için de iki grup arasında istatistiksel açıdan anlamlı bulundu (p<0,05).
Sonuç:
ISR grubunda kontrol grubuna kıyasla serum FSH, LH ve testosteron seviyelerindeki artış, ISR’nin 40 mg/kg dozunun spermatogenezi uyarıcı bir etkiye sahip olduğunu düşündürdü.
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