ÖZET
Amaç:
Atropin ghrelin salınımını azaltmakta, ghrelin ise epileptik nöbetleri baskılamaktadır. Aç hayvanlara atropin uygulanması ve ardından yem verilmesi ile klonik konvülsiyonlar oluştuğunu bildiğimizden bu bilgi dikkat çekicidir. Bu nedenle çalışmamızda, antimuskarinikle indüklenen bu konvülsiyonlar ile ghrelin seviyelerinin ilişkisinin araştırılması amaçlanmıştır.
Yöntemler:
Balb/C fareler 24 saat aç bırakıldı, bu süre sonunda serum fizyolojik veya skopolamin (3 mg/kg, i.p.) uygulandı ve 20 dakika sonra yem verildi. Konvülsiyon oluşumu ve sıklığını belirleyebilmek için tüm hayvanlar 30 dakika süreyle izlendi. Alınan plazma ve beyin dokularında ghrelin seviyeleri ölçüldü.
Bulgular:
Skopolamin uygulanan aç farelerde yem yedikten sonra konvulsiyon oluştuğu izlendi. Serum fizyolojik uygulanan aç hayvanlardaki plazma ghrelin seviyesinin, toklara kıyasla anlamlı derecede yüksek olduğu saptandı. Konvülsiyon geçiren hayvanlardaki plazma ve doku ghrelin seviyelerinin, açlık sonrasında serum fizyolojik uygulanan ve ardından yem verilen hayvanlara göre anlamlı derecede düşük olduğu belirlendi.
Sonuç:
Konvülsiyon geçiren farelerdeki ghrelin düzeylerinin, skopolamin uygulanması ve yem verilmesi sonucunda azaldığı görülmektedir. Çalışmamızdaki bulgular, ghrelinin bu konvülsiyonların oluşumunda rolü olabileceğini düşündürmektedir.
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