ÖZET
Amaç:
Bu çalışmada gemifloksasinin 4-bromometil-7-metoksikumarin belirteci ve dibenz-18-taç-6 eter katalizör varlığında farmasötik preparatlarda yüksek performanslı sıvı kromatografisi ile analizi gerçekleştirilmiştir.
Yöntemler:
Türevlendirme işlemi sonucu oluşan bileşiğin eksitasyon dalga boyu λext. =325 nm ve emisyon dalga boyu λem. =390 nm olarak bulunmuştur. Optimum reaksiyon şartları dikkatlice çalışıldı. Kromatografik ayırmalar, 250x4,6 mm, 5 μm I.D C18 kolonda, asetonitril-0,05 M sulu amonyum asetat (pH=5,0) (70:30, v/v) mobil fazında, 1 mL/dak akış hızında gerçekleştirilmiştir.
Bulgular:
Kalibrasyon eğrisi 10-200 ng.mL-1 aralığında doğrusal bulunmuştur. Ortalama geri kazanım %100,32 ve bağıl standart sapma değerleri %2’nin altında bulunmuştur.
Geliştirilen metod, ilaç maddesin farmasötik preparatlardaki analizine başarıyla uygulanmştır.
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