ÖZET

Amaç:

Kemik doku onarımda altın standart olan otojen kemik greftleri her zaman tüm gereksinimleri karşılayamamaktadır. Bu çalışmanın amacı, otojen yağ doku kaynaklı mezenkimal kök hücrelerin (ATDMSC) ve hücresizleştirilmiş kemik allo greftlerinin kombinasyonunu kullanarak kemik dokusu üretmektir.

Yöntemler:

Tavşan ulnasının 1/3 orta segmentinde oluşturulan 1,5 cm’lik bir kemik defektine, grup tasarımlarına göre, gruplar; 1: kontrol; 2: krio ile muamele edilmiş kemik grefti; 3: hücresizleştirilmiş kemik grefti; 4: ATDMSC implante edilmiş hücresizleştirilmiş kemik grefti olacak şekilde mini plak ve vida ile osteosentez yapıldı.

Bulgular:

Birinci, ikinci ve dördüncü grupta greft iyileşmesi ve düzensiz kallus oluşumu gözlendi. Birinci grupta, haversian sistemlerinin organizasyonu, lakunların yapısı ve canaliculi ossiumların varlığı gözlendi; İkinci grupta, havers kanallarının yaklaşık %40’ı kan damarları içeriyordu ve mikroskobik olarak incelenen alanların %90’ında ince filamentler şeklinde kanalikül ossiumları vardı; Üçüncü grupta, havers kanallarının çoğu boştu, osteosid kanallarının çoğu hücre içermiyordu ve orada kanaliküller ossiumu yoktu; dördüncü grupta, havers kanallarının bir kısmı kan damarları içermekteydi ve hücresizleştirmeye bağlı olarak hücreleri içeren kısmen lakazlar mevcutken, incelenen mikroskop alanlarının yaklaşık %50’sinde, osteositlere farklılaşan mezenkimal kök hücrelerin varlığı ile canaliculi ossium varlığı moleküler testler ile kanıtlandı.

Sonuç:

ATDMSC ile uygun bir niş ortamının oluşturulması halinde kemik greftinin yaşayabilirliğinde artış sağlandı ve bunun ATDMSC’lerin osteojenik hücrelere dönüşüm yoluyla olduğu gösterildi.

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Adipoz Kökenli Kök Hücre Kullanımıyla Kemik Doku Üretimi

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References