Original Article

Resveratrol Attenuates Bleomycin-İnduced Genotoxicity, Pulmonary Fibrosis and DNA Damage in Balb/C Mice with Ehrlich Ascites Carcinoma


  • İsmail KOYUNCU

Received Date: 20.07.2017 Accepted Date: 11.09.2017 Bezmialem Science 2018;6(4):262-271


Oxidative stress plays an important role in the pathogenesis and genotoxicity of bleomycin (BLM)-induced lung fibrosis. The aim of this study was to investigate the protective effect of resveratrol (RES) on lung fibrosis, DNA damage, and genotoxicity associated with BLM in mice with Ehrlich ascites carcinoma (EAT).


A total of 60 BALB/c mice were randomly separated into 6 groups. With the exception of the sham group, mice were injected intraperitoneally (i.p.) with 3×105 live EAT cells, then after 24 hours, mice were administered with BLM only (10 mg/kg, i.p.), RES only (50 mg/kg, i.p.), BLM (10 mg/kg, i.p.)+RES (25 mg/kg, i.p.), and BLM (10 mg/kg, i.p.)+RES (50 mg/kg, i.p.) for 6 days. By examining the oxidant parameters in serum and the lung tissue, such as DNA damage (the comet assay and 8-OHdG), malondialdehyde (MDA), myeloperoxidase (MPO), protein carbonyl (PC), hydroxyproline (HPR), total oxidant status (TOS), and oxidative stress index (OSI), and antioxidant parameters such as superoxide dismutase (SOD), total antioxidant status (TAS), and glutathione (GSH), an evaluation was made of the BLM-related pulmonary toxicity and genotoxicity and the potential role of RES in preventing damage.


The results determined that RES reduced the MDA, PC, TOS, MPO OSI, HPR, and DNA damage levels, which showed an increase related to BLM (p<0.05) and increased GSH, TAS, and SOD activities (p<0.05). It has also been observed that RES inhibits the BLM-induced fibrosis in histological findings.


Our results show that fibrosis that formed as a result of lipid and protein oxidization and DNA damage caused by BLM in the lungs of EAT-carrying mice could be reduced with the application of RES. Therefore, RES could be considered as an adjuvant therapy to conventional treatment methods for pulmonary fibrosis and DNA damage of the lungs.

Keywords: Bleomycin, ehrlich ascites carcinoma, pulmonary fibrosis, resveratrol, DNA damage