Introduction
Despite advanced preventive measures, dental caries still maintains its frequency and importance among oral diseases. Dentists should choose the most appropriate restorative material based on characteristics associated with patient and caries. In addition, physical properties, biocompatibility, esthetic features, and application information of restorative materials help in making the most appropriate choice (1,2).
Conventional glass ionomer cements (GIC) were developed by combining advantages of silicate and polycarboxylate cements. They have advantages such as chemical bonding to dental tissues, releasing and recharging fluoride, compatibility of thermal expansion coefficient with tooth enamel and dentin, and low cytotoxicity. Besides, GICs have disadvantages of a low wear resistance, short working time, long curing time, sensitivity to moisture contamination, and a high rate of microleakage, which limit their usage in restoration of permanent teeth and in areas that will be exposed to an occlusal force in primary teeth (3).
While the first GICs were described as being of a lower viscosity nature, later “high-viscosity” GICs (HVGIC) have been developed to improve insufficient mechanical properties and wear resistance to high occlusal forces of conventional GICs. In addition, they were produced as restorative materials to expand the areas of use restricted to Class I and Class V cavities (3,4).
In order to overcome problems observed in GICs, resin-modified GIC (RMGIC) have been developed (5). Due to resin monomer polymerization, higher resistance to compressive and tensile forces, improved fracture strength, modulus of elasticity, and retention rates are reported with these materials (6,7). Working time of RMCIS is longer than that of conventional GICs. Compared to conventional GICs, RMGICs have disadvantages of weaker adhesions to dental tissues and a lower fluoride release. Increased microleakage due to polymerization shrinkage constitutes further disadvantages of the material (8).
Carbomer-based restoratives contain carbomer fillers and fluoroapatite/hydroxyapatite nanoparticles (9). Glass carbomer (GC) cements are free of monomers like resin, solvent, metal etc. and are easy to diagnose postoperatively due to their radiopacity. Moisture tolerant nature of this material makes it handy in pediatric dentistry. It is stated that pulp capping should not be performed directly with glass carbomer cements (10).
Resistance of a restorative material to intraoral conditions is very important for longevity of restorations. Water sorption and solubility features of restorative materials have a significant impact on clinical success and can not be completely controlled. Water sorption causes dimensional changes in materials, leading to discoloration and a fracture in marginal contours. Water solubility is a phenomenon that adversely affects compatibility of restorations with biological structures and increases rate of deterioration. Studies have shown that sorption and solubility of restorative materials depend on features of solutions (1,11,12).
In order to prevent plaque formation in children aged ≥6 years, supervised use of fluoride-based mouthrinses as well as toothpastes prevents demineralization of tooth structure and provides remineralization of early caries lesions (13).
Few studies have been conducted to evaluate effects of oral mouthrinses on the water sorption and solubility properties of restorative materials. Mouthrinses contain different concentrations of water, antimicrobial agent, salt, preservatives, and alcohol. In particular, alcohol has been reported to cause increased wear of the material (14,15).
In this study, our purpose was to compare water sorption and solubility values of GC (GCP Glass Carbomer Cement), HVGIC (Riva Self Cure HV), and RMGIC (Riva Light Cure HV) restorative materials in mouthrinse with alcohol (Listerine Cool Mint), mouthrinse without alcohol (Listerine Total Care Zero), and artificial saliva. The null hypothesis stated that water sorption and solubility values do not differ according to the restorative material or solvent type tested.
Method
Sample Preparation
Restorative materials used in the present study and polymerization types recommended by the manufacturer are shown in Table 1 and the solutions used in the study are shown in Table 2. Samples were prepared for each material using circular teflon molds with a diameter of 10 mm and a thickness of 2 mm. The mold was isolated with petroleum jelly to prevent the materials from sticking to the mold. The materials were mixed in amalgamators at room temperature according to the manufacturer’s instructions and were placed into the molds. Transparent matrix strips (Universal Strips, Extreme Dental, İstanbul, Turkey) were placed on the upper surface of the molds to overflow the surplus. The samples were polymerized according to the manufacturer’s instructions. After the hardening of the material, the molds were carefully removed and the debris around them was cleaned. Twenty-one samples were prepared for each material group, which were divided into three sub-groups of seven to be placed in different solution media.
Sorption and Solubility Measurements
All samples were stored in a desiccator with an anhydrous self‑indicating silica gel at 37±1 ºC for 24 hours. Then, the initial weights of the samples were measured on an electronic analytical balance (brand) as micrograms (mg) and recorded as 𝑚L. The samples were suspended in different solutions according to their groups for 24 hours at 37 °C. Subsequently, the samples were removed from the solutions and their weights were measured one minute after removal and recorded as 𝑚2. After the measurement process, the samples were placed again in the desiccator for 24 hours to evaporate the water content and, subsequently, their weights were measured and recorded as 𝑚3. The volumes of the samples were found in mm3 according to the formula 𝑉=𝜋xr2xℎ, where r is the radius of the average diameter/2 and ℎ is the average thickness. Then, the water sorption and solubility of the samples were found in mg/mm3 with the formulas 𝑚2 - 𝑚3/V and 𝑚1 - 𝑚3/V, respectively.
Statistical Analysis
The IBM SPSS Statistics 20 program was used to perform statistical tests with a significance level set at 5%. Besides, descriptive statistics, one-way ANOVA, and post-hoc Tukey tests were used to compare means of sorption and solubility between the groups.
Results
The mean water sorption and solubility values for each material and medias used in the study are shown in Tables 3 and 4, respectively. Also, in Figure 1, both the mean water sorption and solubility values were presented for each material at different media.
The glass carbomer materials used in the study were mostly found to be fragmented. Only 1, 3, and 2 sound samples were left to be measured for the artificial saliva, mouthrinse with alcohol and mouthrinse without alcohol groups, respectively. Although the results of this group are shown in the tables, they were not included in the statistical analysis due to the small number of samples.
Water sorption values were found to be significantly higher for the RMGIC group compared to the HVGIC group in all the three different media (p<0.001). HVGIC material showed similar water sorption values for all the three media (p=0.123). However, RMGIC material showed the highest sorption in artificial saliva, which was followed by the mouthrinse without alcohol and mouthrinse with alcohol, respectively (psaliva-with alcohol <0.001, psaliva-without alcohol =0.001, pwith alcohol-without alcohol <0.001).
Solubility values were significantly lower in the HVGIC group compared to the RMGIC group in artificial saliva (p<0.001) and mouthrinse without alcohol environments (p<0.001), but no significant difference was found in the mouthrinse with alcohol environment (p=0.114). While RMGIC showed similar solubility in all three environments (p=0.480), the HVGIC material showed significantly lower solubility in artificial saliva compared to other media (psaliva-with alcohol <0.001, psaliva-without alcohol =0.006, pwith alcohol-without alcohol =0.206).
Discussion
Due to esthetic properties, polymer-based materials are used in dental restorations, but hydrophilic properties of these materials result in some degree of water sorption or dissolution in these materials (16). Water sorption and dissolution have been reported to adversely affect the clinical success of these restorations (17,18).
Conventional GICs are restorative materials that can easily draw water into their structures. It is very necesaary to investigate water sorption and solubility levels of restorative materials in order to increase clinical success rate of restorations and determine their application areas (19). In conventional GIC, water sorption occurs primarily within the matrix. Due to the water sorption, hydrolysis of the cement matrix occurs. Cement mass deteriorates over time and loss of surface properties, marginal integrity, esthetic appearance, and consequent deterioration in restorations occur (5). To overcome well-known disadvantages of GICs, RMGICs and high-viscosity GICs have been developed. While there are many studies evaluating the physical properties of high-viscosity GICs in the literature, the studies evaluating water sorption and solubility are limited (11,20-22). In previous studies, the sample dimensions were chosen to be 10-15 mm in diameter and 1-4 mm in thickness. Sample sizes are effective in diffusion of water to the polymer matrix. Smaller sample size shortens stabilization time in the material (23,24). The diameter of the specimens used in our study was prepared as 10 mm according to diameter of the tip of the light curing device, and the thickness was selected as 2 mm according to the layering method. There are studies with just one-hour storage solutions as well as one-year storage solutions to assess water sorption and solubility (25,26). Residence time is known to affect water sorption and solubility levels of the materials. Since it is known that pH changes may affect diffusion and solubility, the residence time used in our study was chosen as 24 hours to prevent variations in pH. In order to determine water sorption and solubility of the materials, different formulas are used in different studies (27-34). We used the formulas prepared under the guidance of the ISO standard in our study. All these methodological differences (sample size, solution storage time, formulation of the water sorption, and solubility) may have a role in the result inconsistencies in the literature.
RMGIC materials have been reported to exhibit higher water sorption and solubility than composites (35). It has also been reported that RMGIC materials have a higher and faster water sorption than conventional GIC materials (28). In another study, RMGIC materials showed similar or higher sorption values than HVGIC materials (33).
It has been claimed that high water sorption of RMGIC is caused by the initial desiccation procedure, which disrupts acid-base reactions (30). Another suggestion is that polycarboxylic acid, inorganic glass particles, and HEMA contained in RMGIC structure retain large amounts of water (28). In our study, based on existing literature, the water sorption values were significantly lower in all the three solution environments for the HVGIC group compared to the RMGIC group. In solubility values, it was observed that the HVGIC group showed less dissolution than the RMGIC, except in the alcohol-free mouthrinse group. With increasing concerns about more effective oral hygiene habits, particularly with regard to the pediatric population, the use of chemical control agents (e.g., mouthwashes) has been adopted to complement toothbrushing and dental flossing (36). Their composition is based on water, antimicrobial agents, salts, preservatives, alcohol, and hydrogen peroxide (37). It has been reported that mouthrinses, containing alcohol or not, can increase the sorption and solubility for restorative materials compared to distilled water, but this effect may vary according to the material tested (29,31,32). In our study, the HVGIC material showed a significantly lower solubility in the artificial saliva environment than in other environments as expected, because mouthwashes trigger a decrease in the oral pH, which has been associated with an increase in the solubility of dental materials (38,39). However, RMGIC showed similar solubility in all the three environments, which may be explained by its already high solubility in artifical saliva due to its hydrophilicity. The sorption values of the RMGIC material were higher in the mouthrinse without alcohol environment than in the mouthrinse with alcohol environment. Also in artificial saliva environment RMGIC showed highest sorption values. These results may be due to the rapid water sorption of HEMA, a significant resin component found in the RMGIC material. In the HVGIC group, there was no difference between the two mouthrinses and artificial saliva environments in terms of sorption. Contrary to previous studies (37,40), mouthrinses did not increase the sorption of the HVGIC materials used in the present study, which may be explained by the more resistant structure of these materials to the chemical ingredients of the mouthrinses (41,42).
Savas et al. (33) and Subramaniam et al. (34) evaluated water solubility of GC in their study and reported that it is lower than conventional GICs. They did not mention fragmentation as in our study (33,34). In the present study, after the fragmentation of GC samples, new specimens were prepared by applying a surface covering on the GC materials, but sample loss due to serious fragmentation was experienced again.
Subramaniam et al. (34) modified the section 7.12 of ISO 4049 by placing specimens in a solution of artificial saliva immediately after preparation. They claimed that desiccation might affect the glass ionomer specimens’ water sorption and solubility results due to damage (30). The reason for the disintegration of our GC samples may be bacause they were placed in a desiccator immediately after curing and removal from the mold as described in section 7.12 of ISO 4049.
Study Limitations
The main limitations of this study are in vitro design and the use of arguably shorter time periods to test water sorption and solubility. Clinical studies must be conducted to confirm the results.
Conclusion
High-viscosity GIC and RMGIC materials show significant water sorption and solubility; especially, RMGIC materials perform poorly in terms of sensitivity to water and this may cause degradation in the oral environment. Whether it contains alcohol or not, mouthwashes may have an adverse effect on the material structure by increasing sorption of RMGICs and solubility of GICs.
Further long-term studies are needed to investigate the sorption and solubility characteristics of these fluoride-releasing restorative materials.
Peer-review: Externally peer reviewed.
Authorship Contributions
Surgical and Medical Practices: E.B., S.B.K., Concept: N.Ö., Design: N.Ö., Data Collection or Processing: N.Ö., E.B. Analysis or Interpretation: N.Ö. Literature Search: N.Ö., S.B.K., Writing: N.Ö.
Conflict of Interest: No conflict of interest was declared by the authors.
Financial Disclosure: The authors declared that this study received no financial support.